RBC Genomic DNA Extraction Kits for Blood/Bacteria/Cultured Cells provides a fast and economical method for purification of total DNA (including genomic, mitochondrial and viral DNA) from whole blood, plasma, serum, buffy coat, other body fluids, lymphocytes and cultured cells. This kit is also ideal for bacteria and cultured cells. The blood protocol utilises RBC lysis buffer, GB buffer and a rapid heating step to release DNA into solution. DNA in the chaotropic salt solution binds to the glass fiber matrix of column. After washing off the contaminants, the purified DNA is eluted by low salt elution buffer or water. There is no requirement for phenol/chloroform extraction or alcohol precipitation.
RBC Genomic DNA Extraction Mini Kit for Tissue has been designed for purification of total DNA (including genomic, mitochondrial) from a variety of animal tissues or cells. The provided micropestle can efficiently homogenize tissue sample to shorten the time for lysis. The method uses proteinase K and a chaotropic salt, guanidine hydrochloride to lyses cells and degrade protein. DNA in chaotropic salt binds to the glass fiber matrix of column. After washing off the contaminants, the purified DNA is eluted by low salt elution buffer or water.
RBC Genomic DNA Extraction Kit for Plants (Duo Buffer System) provides a fast and simple method to isolate DNA from plant tissue and cells. In the first step the sample is lysed by homogenization. The lysate is treated with RNase A to remove RNA. In the presence of a chaotropic salt, the genomic DNA in the lysate binds to the glass fiber matrix in the spin column. The contaminants are washed with an ethanol based wash buffer and purified genomic DNA is eluted by low salt elution buffer or water. The protocol does not require DNA phenol extraction and alcohol precipitation. The entire procedure can be completed in 60 minutes.
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